Analysis Note

ControlRAW264.7 cells

Application

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

Anti-Tim3 Antibody, clone 5D12 is an antibody against Tim3 for use in FC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

As a member of the T cell immunoglobulin and mucin domain (TIM) family, Tim-3 is reported to promote or terminate Th1 immunity and is thought to influence a range of inflammatory conditions. It is constitutively expressed in a subset of murine IFNgamma-secreting Th1 cells, but not Th2 cells, in both human and mice. Additionally, Tim-3 is expressed dendritic cells, and by a subset of CD4+ cells where its levels of expression increase upon anti-CD3/28 stimulation. This molecule is also be highly expressed in in vitro polarized Th1 cells, and at lower levels in Th17 cells. Tim-3 is known to synergize with Toll-like receptors (TLRs) and is thought to regulate Th1 and Th17 cytokine secretion.

Immunogen

Recombinant protein corresponding to full length mouse Tim3.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Flow Cytometry in RAW264.7 cells.

Flow Cytometry Analysis: A 1:50 dilution of this antibody detected Tim3 in RAW264.7 cells.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

31 kDa calculated